The Use of DNA Barcoding and Phylogenetic Analysis to Improve Identification of <i>Usnea</i> spp. Based on ITS rDNA

Lichen of the genus Usnea is quite common being used as a traditional herbal remedy. This characterized by thallus, which very similar among species, leads to some difficulties in distinguishing them. In Indonesia, such research report on availability this based their morphological characteristic minimal. might be due too high similarities The molecular character, DNA Barcode Internal Transcribed Spacer (ITS) rDNA sequences, with its conserved region (5.8S) and varied (ITS1 ITS2), are becoming essential characters identifying well analyzing phylogenetic. current study then proposed identify draw species dendrogram within obtained from Mount Lawu Forest Central Java Turgo Yogyakarta phylogenetic phenetic analysis. was constructed UPGMA using simple matching coefficient, whereas tree Maximum Likelihood (ML) Kimura-2 parameter 1000 bootstrap. data were unable relationships subgenus Eumitria members. shows primary two clades, Eumitria. ITS sequence able most species.